Comparison of droplet digital PCR and quantitative real-time PCR in mcrA-based methanogen community analysis
نویسندگان
چکیده
منابع مشابه
Comparison of droplet digital PCR and quantitative real-time PCR in mcrA-based methanogen community analysis
Two different quantitative PCR platforms, droplet digital PCR (dd-PCR) and quantitative real-time PCR (qPCR), were compared in a mcrA-based methanogen community assay that quantifies ten methanogen sub-groups. Both technologies exhibited similar PCR efficiencies over at least four orders of magnitude and the same lower limits of detection (8 copies μL-DNA extract-1). The mcrA-based methanogen c...
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Employment or Leadership: C.R.L. Teo, National University of Singapore; S.S. Chong, National University of Singapore. Consultant or Advisory Role: None declared. Stock Ownership: None declared. Honoraria: None declared. Research Funding: C.G. Lee, National Medical Research Council; S.S. Chong, National Medical Research Council. Expert Testimony: None declared. Patents: C.R.L. Teo, patent number...
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ژورنال
عنوان ژورنال: Biotechnology Reports
سال: 2014
ISSN: 2215-017X
DOI: 10.1016/j.btre.2014.06.010